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We have achieved 1.5 nm resolution using fluorescence imaging, approximately 300 times better than the diffraction limit of conventional light. Recently we have been able to increase the time resolution to 1 msec, from a previous value of 500 msec. Using this increased time-resolution, we have looked at molecular motors inside living cells. We have been able to see individual cargos being moved by individual kinesin and dynein, two important motors. We find that both kinesin and dynein move cargo 8 nm per ATP (the universal food of the cell), in opposite directions in a cell. Amazingly, these two molecular motors do not engage in a tug-of-war, but appear to be cooperative, giving the particle extra speed.(click here for webcast) |
