Physics @ Berkeley
Events in Physics
Title: Fluorescence Imaging with One Nanometer Accuracy (FIONA): Application to Molecular Motors    (Colloquia)
Start Date: 02/28/2005
Time: 4:30 pm
Location: 1 LeConte Hall
Speaker: Professor Paul R. Selvin
Affiliation: Dept of Physics, Univ of Illinois at Urbana-Champaign
Contact Person: K. Lee   (510) 642-3034
Details: We have achieved 1.5 nm resolution using fluorescence imaging, approximately 300 times better than the diffraction limit of conventional light. Recently we have been able to increase the time resolution to 1 msec, from a previous value of 500 msec. Using this increased time-resolution, we have looked at molecular motors inside living cells. We have been able to see individual cargos being moved by individual kinesin and dynein, two important motors. We find that both kinesin and dynein move cargo 8 nm per ATP (the universal food of the cell), in opposite directions in a cell. Amazingly, these two molecular motors do not engage in a tug-of-war, but appear to be cooperative, giving the particle extra speed.

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