Laser phase plate for transmission electron microscopy
Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, can benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging of all such devices has hindered these efforts. Mueller and team have demonstrated electron phase manipulation with a high-intensity continuous-wave laser beam, and use it as a phase plate for TEM. These results suggest the possibility for dose-efficient imaging of unstained biological macromolecules and cells. Read the entire article in Nature Methods.